Semester 1 SMBB This course encompasses the basic principles and techniques involved in molecular biology which will enable students to apply these techniques in the genetic engineering laboratory. The introductory lecture will expose students to genetic engineering and its application in various sectors of the industry such as agriculture, medical, pharmaceutical, environmental, etc.
They involve slowly adding a reagent to a reaction mixture until the chemical reaction is complete. The completion of the reaction is usually marked by the color change of an indicator substance. The volume of reagent required to complete the reaction is precisely measured using a burette.
Calculations can then be carried out to determine the concentration of the original substance. Complete your titration ensuring you achieve concordant results. You should have three results within 0. For a titration, the introduction should include information about what you hope to find out and what substance or product you will be analyzing.
Write about the reaction you will be using, including the equation and the conditions required. Include details of the indicator stating the expected color change and writing a brief explanation of the suitability of the chosen indicator.
Sciencing Video Vault Describe details of your experimental method in the next section. Include a description of how you made up your solutions, if applicable.
State the volume and concentration of any reagents used. Draw a table to represent the results of your titration. It is customary to write the final burette volume in the first row, the initial burette volume in the second row and the titre in the third row. The titre is calculated by subtracting the initial volume from the final volume.
To indicate precision, write all your results in cubic centimeters to two decimal places, adding a zero to the end of the number if necessary. Most standard burettes allow measurement to the nearest 0. Include all your repeat readings in the table, and indicate which are the concordant results to be used in the calculation of the mean titre.
Calculate the mean titre using the concordant results only and record it below your results table. Calculate your unknown using the mean titre and standard volumetric analysis methods. Lay out your calculations clearly, writing them down in a step-by-step format.
This will help you to avoid mistakes, and will also ensure you are given credit for method if you make a minor error.
Ensure you add the appropriate units to your answers, and use a suitable degree of precision: For guidance on completing the calculations, there are a number of online resources.
In a titration, the conclusion is often a simple statement of the experimentally determined parameter. Depending on the aim of the titration, more detail may be required. For example, a brief discussion on whether the results fall within the expected range may be appropriate.Acid-base titrations can also be used to quantify the purity of chemicals.
Acid-base titration The solution in the flask contains an unknown number of equivalents of base (or acid). The burette is calibrated to show volume to the nearest cm 3. An acid-base titration is the process whereby you determine the volume of standard solution required to neutralize a solution of acid or base of unknown concentration.
In these titrations, an indicator, such as phenolphthalein or cresol red, is used to determine the end-point of the titration. AET Internal Combustion Engine Theory and Servicing.
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The purpose of biodiesel titration is to determine the amount of catalyst needed to neutralize the unknown amount of acid (FFA) in waste vegetable oil (WVO).
This biodiesel preparation process will determine how much of the catalyst (a .